Fig. 5

mRpL4 physically and genetically interacts with wap A. Representative western blotting (n = 3 biological repeats) of lysates from wild‐type and UAS‐mRpL4‐RNAi‐expressing wing disks. The custom mRpL4 antibody was used and the β‐tubulin protein was included as loading control. B. Representative immunoprecipitation analysis (n = 3 biological repeats) using lysates from wing disks overexpressing HA‐tagged wap. Anti‐HA antibodies were used for immunoprecipitation. Western blotting was performed using anti‐HA and anti‐mRpL4 antibodies to reveal wap and mRpL4, respectively. GAPDH was used as control. C. Representative image of wings (n > 15 wings) from flies expressing UAS‐wap‐RNAi under the control of hh‐Gal4. D–F. Representative images of wing disks (n > 15 wing disks) from flies expressing UAS‐wap‐RNAi under the control of hh‐Gal4 that have been stained for Cut (D), E(spl)mβ‐LacZ (E) and DHE (F). G. Representative image of wing disks (n > 10 wing disks) bearing MARCM clones (marked by GFP) stained for Wg. In the mRpL4K14608 MARCM clones, UAS‐HA‐wap are expressed under the control of tub‐Gal4. H. Representative image of wings (n > 15 wings) from flies expressing UAS‐mnb‐RNAi under the control of hh‐Gal4. I. Representative image of wing disks (n > 15 wing disks) from flies expressing UAS‐mnb‐RNAi under the control of hh‐Gal4 that have been stained for Cut.