Fig. 2
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mRpL4 regulates Notch signaling in the Drosophila wing A. Representative image of wings (n > 20 wings) from flies expressing mRpL4 RNAi, UAS‐mRpL4 and both under the control of nub‐Gal4. B. Representative image of wing imaginal disks (n > 15 wing disks) stained for Cut, from flies expressing mRpL4 RNAi, UAS‐mRpL4 and both under the control of nub‐Gal4. C. Representative images of wing disks (n > 15 wing disks) stained for Cut and Wg from flies expressing UAS‐mRpL4‐RNAi under the control of hh‐Gal4 (marked by GFP). D. The level of Su(H) occupancy at E(spl)mβ gene family regions as assessed by qPCR following ChIP, from wild‐type and UAS‐mRpL4‐RNAi‐expressing wing disks. Data are presented as mean ± SEM, two biological replicates for each genotype and three technical replicates for each sample. Statistical significance was tested using two‐tailed unpaired t‐test. *P < 0.05, **P < 0.01. |