Fig. 8.

α-Islet1 antibody and sost:nlsEos label differentially label regenerating hair cells. Confocal projections of neuromasts showing hair cells labeled with myo6:GFP (green), central cells and hair cells labeled with α-Isl1 antibody (red), and sost:nlsEos cells (blue). Examples of hair cells that are both myo6:GFP+ and sost:nlsEos are marked with cyan arrows, hair cells that are both myo6:GFP+ and α-Isl1+ are marked with yellow arrowheads, hair cells that are myo6:GFP+, α-Isl1+, and sost:nlsEos+ are marked by white arrows, and cells that are α-Isl1+ and sost:nlsEos+ are marked with purple asterisks. (A) Timeline of UV photoconversion of sost:nlsEos cells at 5 dpf, homeostasis for 3 days, and fixation at 8 dpf. (B-C′″) Heterozygous sibling and foxg1^a266 mutants neuromasts at 8 dpf. (Dⁱ-D^vii) Quantification of myo6:GFP+ hair cells (Dⁱ), α-Isl1+ cells (Dⁱⁱ), sost:nlsEos+ cells (Dⁱⁱⁱ) cells labeled with both myo6:GFP and α-Isl1 (D^iv), hair cells labeled with myo6:GFP and sost:nlsEos (D^v), cells labeled with sost:nlsEos and α-Isl1 (D^vi), and cells labeled with myo6:GFP, α-Isl1, and sost:nlsEos (D^vii), n=15 neuromasts (eight larvae) heterozygous siblings and 14 neuromasts (eight larvae) foxg1^a266 mutants. (E) Timeline of UV photoconversion of sost:nlsEos cells and NEO exposure at 5 dpf, regeneration for 3 days, and fixation at 8 dpf. (F-G′″) Heterozygous sibling and foxg1^a266 mutants neuromasts at 8 dpf. (Hⁱ-H^vii) Quantification of myo6:GFP+ hair cells (Hⁱ), α-Isl1+ cells (Hⁱⁱ), sost:nlsEos+ cells (Hⁱⁱⁱ) cells labeled with both myo6:GFP and α-Isl1 (H^iv), hair cells labeled with myo6:GFP and sost:nlsEos (H^v), cells labeled with sost:nlsEos and α-Isl1 (H^vi), and cells labeled with myo6:GFP, α-Isl1, and sost:nlsEos (H^vii), n=15 neuromasts (eight larvae) heterozygous siblings and 14 neuromasts (nine larvae) foxg1^a266 mutants. All data presented at mean±s.d. Significance was determined with Mann–Whitney U-tests. Scale bars: 20 µm.