FIGURE

Fig. 6

ID

ZDB-FIG-240910-9

Publication

Bao et al., 2024 - A UTP3-dependent nucleolar translocation pathway facilitates pre-rRNA 5'ETS processing

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Fig. 6

The C-terminus of UTP3 interacts with KPNA3. (A) Diagram showing predicted Nuclear Localization Sequences (NLSs), determined via the NLS Mapper tool, with a set cut-off score of 5. MP-NLS, predicted monopartite NLS; BP-NLS, predicted bipartite NLS. (B) Western blot of the Co-IP products pulled down using the FLAG antibody (FLAG was tagged to the KPNA3) for analyzing the interaction of KPNA3 with different UTP3 derivatives as indicated. TUBULIN was used as a loading control for the input. Star denotes unspecific bands. (C) Immunostaining of bimolecular fluorescence complementation experiment. Co-immunostaining of KPNA3 with UTP3, UTP3 derivatives or vector in HeLa cells 48 h after transfecting with corresponding plasmids. KPNA3 were detected using an HA antibody, UTP3 derivatives or vector were detected using a MYC antibody. Scale bar, 5 μm. (D) Live cell images of the bimolecular fluorescence complementation system were captured 48 h post-transfection. The plasmids used were indicated in the figure panels. The magnification view of black box is shown on the right. Scale bar, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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