FIGURE

Fig. 10

ID

ZDB-FIG-240910-13

Publication

Bao et al., 2024 - A UTP3-dependent nucleolar translocation pathway facilitates pre-rRNA 5'ETS processing

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Fig. 10

UTP3 directly interacts with and mediates the nucleolar localization of EXOSC10. (A) Western blot showing the Co-IP product pulled down using an HA antibody (HA fused to EXOSC10). TUBULIN was used as the loading control. (B) Representative images showing the effect of knockdown of the endogenous UTP3 on nucleolar localization of FBL, EXOSC10, MTR4 and DIS3 in HeLa cells. The shLUC was used as a negative control. Scale bar, 5μm. Arrow in each panel indicates a representative UTP3 knockdown cell. (C) Western blot of the Co-IP products pulled down using the FLAG antibody (FLAG was tagged to derivatives of EXOSC10) for identifying the interaction domain between EXOSC10 and UTP3. Co-IP result showed that UTP3 interacted with the C-terminal region of EXOSC10 (E10601-885), as indicated. (D) GST-pull down experiment showing the direct interaction between UTP3 and EXOSC10. Recombinant GST (black arrow), GST-UTP3 (yellow arrow) and HIS-EXOSC10601-885 (red arrow) were expressed and purified. Since GST-UTP3 lane displayed a non-specific band of similar size to HIS-EXOSC10601-885, the flowthrough (FT) fraction after GST resin incubation was run in parallel to show that the EXOSC10601-885 was retained by the beads when being co-incubated with GST-UTP3 but not with GST.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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