Fig. 2
Nucleolar localization of the endogenous MPP10, UTP13, UTP25 and EMG1 is UTP3-dependent. (A–H) Representative images showing the effect of knockdown of the endogenous UTP3 on nucleolar localization of FBL (A), NPM1 (B), MPP10 (C), UTP13 (D), UTP25 (E), EMG1 (F), UTP18 (G) and BMS1 (H) in HeLa cells. The endogenous UTP3 protein was knocked down using the DOX-inducible UTP3-specific shRNA (shUTP3). The LUC shRNA (shLUC) was used as a negative control. Immunofluorescence staining using antibodies against FBL, NPM1, UTP3, MPP10, UTP13, UTP25 and EMG1 were performed 96 h after DOX treatment. Nuclei (blue) were stained with DAPI. Scale bar, 5μm. Arrow in each panel indicates a representative UTP3-knockdown cell. |