Fig. 4

Peds1 deficiency hampers tissue regeneration.

A Schematic of the experimental procedure used for tail injury and regeneration assays. Three dpf larvae previously microinjected with std or peds1 crRNA/Cas9 complexes alone or in combination with cril1b were tail fin amputated and regeneration was evaluated by measuring regenerated fin areas up to 5 dpf (yellow). B, C Measurement of regenerated fin areas and representative images (brightfield channel). D Analysis and representative images of Il1b fluorescent reporter expression levels (green channel; Tg(il1b:GFP-F)^ump3) by microscopy fluorescence. Each point represents one larva and the mean ± SEM of each group is also shown. P-values were calculated using one-way ANOVA and Tukey’s multiple range. n.s, not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001. auf arbitrary units of fluorescence.