FIGURE

Fig. 6

ID

ZDB-FIG-240703-113

Publication

He et al., 2024 - Meningeal lymphatic supporting cells govern the formation and maintenance of zebrafish mural lymphatic endothelial cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

The muLEC-derived lymphatic vessels perform the functions of transporting immune cells and draining cerebrospinal fluid.a Dorsal confocal images of lyz:GFP+ lymphocytes in DMSO-treated brains at 20 dpf/14 dpt. n = 6. b Time-lapse images of the flow of lyz:GFP+ lymphocytes in mural lymphatic endothelial cell (muLEC)-derived lymphatic vessels of Mtz-treated brains at 20 dpf/14 dpt. The duration of time-lapse imaging is represented in hours:minutes. n = 6. The experiment was repeated three times independently with similar results. c Percentage of fish whose muLEC-derived vessels with (green) and without (gray) immune cells. 10 fish were observed in three independent experiments in each group. d, e, l Illustrations of intracerebroventricular (ICV) and dorsal aorta (DA) injection points of Alexa647-dextran and image areas. f, g Confocal images of the uptake of Alexa647-dextran by muLECs in meningeal lymphatic supporting cell (mLSC)-uninjured brains after ICV injection. n = 20 per experiment. Each experiment was repeated three times independently with similar results. h Confocal images of the uptake of Alexa647-dextran by muLEC-derived lymphatic vessels in mLSC-injured brains after ICV injection. n = 17. The experiment was repeated three times independently with similar results. i, j Confocal images of the uptake of Alexa647-dextran by blood vessels in mLSC-uninjured brains after DA injection. n = 14 per group. Each experiment was repeated three times independently with similar results. k Confocal images of the flow of Alexa647-dextran in blood vessels but not muLEC-derived lymphatic vessels in mLSC-injured brains after DA injection. n = 14. The experiment was repeated three times independently with similar results. m, n Quantification of the number of muLECs endocytosed dye as a percentage of total muLECs (m) and the length of muLEC-derived vessels draining fluid as a percentage of total muLEC-derived vessels (n) after ICV or DA injection of Alexa647-dextran. 12 fish were observed in three independent experiments in each group. Error bars, mean ± SEM. Unpaired two-tailed Student’s t-test. P values included in the graphs. Source data are provided as a Source Data file. The white dashed boxes outline the enlarged areas. Scale bars: 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.