Fig. 7

YAP subcellular localization is associated to autophagy activity in vivo.

a, b Representative images (a) and quantification (b) of RFP-LC3 puncta in Wt1b-GFP⁺ pronephros, at 24 h post fertilization (hpf) and 48 hpf. Data show the mean ± s.e.m.; n = 18 individual data points from 3 independent experiments, two-sided t-test. Scale bars, 10 µm. c, d Representative images (c) and quantification (d) of YAP nuclear localization in Wt1b-GFP⁺ pronephros at 24 and 48 hpf. Nucleus were labeled with Hoechst. Data show the mean ± s.e.m.; n = 60 individual data points from 3 independent experiments, two-sided t-test. Scale bars, 10 µm. e, f Mice subjected to unilateral ureteral obstruction (UUO) or sham operation were euthanized 24 h after surgery and kidney sections subjected to immunohistochemistry for YAP and renal tubule marker Wheat Germ Agglutinin (WGA). e Representative images. Scale bars, 10 µm. f Quantification of YAP nuclear levels in the renal tubules (WGA⁺). Data show the mean ± s.e.m.; n = 5 (sham) and n = 6 (UUO) different mice. two-sided t-test. Source data are provided as a Source Data file.