Fig. 2

The loss of YAP or TAZ stimulates the autophagy flux during shear stress.

a, b Representative images (a) and quantification (b) of LC3 puncta in KECs after transfection with a control siRNA (si Scr) or siRNA against YAP, then subjected to flow (shear) or not (static) during 24 h in the presence or absence of chloroquine (CQ). #: number. c, d Representative images (c) and quantification (d) of LC3 puncta in KECs after transfection with a control siRNA (si Scr) or siRNA against TAZ, then subjected to flow (shear) or not (static) during 24 h in the presence or absence of chloroquine (CQ). e Representative images of YAP, LC3-I, LC3-II and Actin proteins levels in KECs after transfection with a control siRNA (si Scr) or siRNA against YAP, subjected to flow (shear, 24 h) or not (static), in the presence or absence of chloroquine (CQ), by western blot analysis. f The ratio of LC3-II to Actin was determined by densitometry, relative to panel e. g Representative images of TAZ, LC3-I, LC3-II and Actin proteins levels in KECs after transfection with a control siRNA (si Scr) or siRNA against TAZ, subjected to flow (shear, 24 h) or not (static), in the presence or absence of chloroquine (CQ), by western blot analysis. h The ratio of LC3-II to Actin was determined by densitometry, relative to panel g. i, j Representative images (i) and quantification (j) of mRFP-GFP-LC3 puncta in KECs transfected with the mRFP–GFP–LC3 tandem probe, subjected to shear stress during 24 h. k Representative images of YAP, LC3-I, LC3-II and Actin proteins levels in WT (YAP^WT) and (YAP^KO) KECs (CRISPR Cas9) subjected to flow (shear, 24 h) or not (static), in the presence or absence of chloroquine (CQ), by western blot analysis. l The ratio of LC3-II to Actin was determined by densitometry, relative to panel k. b, d, j Data show the mean ± s.e.m.; n = 6 (b, d) or n = 20 (j) from 3 independent experiments, two-sided t-test. Scale bars, 10 µm. f, h, l Data show the mean ± s.e.m.; n = 3 independent experiments, two-sided t-test. Source data are provided as a Source Data file.