FIGURE

Fig. 1

ID

ZDB-FIG-231208-34

Publication

Claude-Taupin et al., 2023 - The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells

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Fig. 1

Physiological shear stress inhibits YAP/TAZ.

a, b Representative images (a) and quantification (b) of YAP localization in the nucleus, labeled with DAPI, in KECs subjected to flow (shear) or not (static) during 24 h. Data show the mean ± s.e.m.; n = 3 independent experiments, two-sided t-test. Scale bars, 10 µm. c, d Representative images (c) and quantification (d) of TAZ nuclear localization in KECs subjected to flow (shear) or not (static) during 24 h. Data show the mean ± s.e.m.; n = 3 independent experiments, two-sided t-test. Scale bars, 10 µm. e Luciferase assay for YAP/TAZ activity in KECs subjected to shear stress or not (static) during 24 h. Data show the mean ± s.e.m.; n = 6 from 3 independent experiments, two-sided t-test. f, g Expression of Cyr61 (f) and Ankrd1 (g) in KECs subjected to flow (shear) or not (static) during 24 h. mRNA levels were quantified by real-time RT-qPCR, normalized to β-actin and are presented as fold increases. Data show the mean ± s.e.m.; n = 3 independent experiments, two-sided t-test. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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