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YAP/TAZ inactivation by shear stress requires a functional primary cilium. a, b Representative images (a) and quantification (b) of YAP nuclear localization in KECs after transfection with a control siRNA (si Scr) or siRNA against KIF3A, subjected to flow (shear) or not (static) during 24 h. Data show the mean ± s.e.m.; n = 3 independent experiments, two-sided t-test. Scale bars, 10 µm. c, d Representative images (c) and quantification (d) of TAZ nuclear localization in KECs after transfection with a control siRNA (si Scr) or siRNA against KIF3A, subjected to flow (shear) or not (static) during 24 h. Data show the mean ± s.e.m.; n = 3 independent experiments, two-sided t-test. Scale bars, 10 µm. e Luciferase assay for YAP/TAZ activity in KECs subjected to shear stress or not (static) during 24 h, after a knockdown of KIF3A. Data show the mean ± s.e.m.; n = 9 from 3 independent experiments, two-sided t-test. f, g Representative images (f) and quantification (g) of YAP nuclear localization in KECs after transfection with a control siRNA (si Scr) or siRNA against CEP164, subjected to flow (shear) or not (static) during 24 h. Data show the mean ± s.e.m.; n = 6 from 3 independent experiments, two-sided t-test. Scale bars, 10 µm. h Luciferase assay for YAP/TAZ activity in KECs subjected to shear stress or not (static) during 24 h, after a knockdown of CEP164. Data show the mean ± s.e.m.; n = 6 from 3 independent experiments, two-sided t-test. Source data are provided as a Source Data file.
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