FIGURE

Fig. 8

ID

ZDB-FIG-230913-18

Publication

Simonian et al., 2023 - Methylenetetrahydrofolate reductase deficiency and high dose FA supplementation disrupt embryonic development of energy balance and metabolic homeostasis in zebrafish

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Fig. 8

FA dose and mthfr genotype interact to affect metabolic outcomes. (A) Calculated mean gray area of viscera region at 5 dpf in WT and mthfr −/− mutants (mthfr −/−, HSC194) exposed to either 0 or 100 mm FA from 0 to 5 dpf and stained with ORO. (B) Relative metabolic rate at 5 dpf of WT and mthfr −/− exposed to either 0 or 100 mm FA from 0 to 5 dpf. (C) Number of NR+ cells at 4 dpf WT and mthfr −/− exposed to either 0 or 100 mm FA from 0 to 4 dpf. (D) Relative food intake, measured by CTCF, at 5 dpf in WT and mthfr −/− exposed to either 0 or 100 mm FA from 0 to 5 dpf. All values are mean ± SEM. Analyzed by two-way ANOVA with FA dose (0 versus 100 mm) and mthfr genotype (WT versus mthfr −/−) as main factors and a Dose × Genotype interaction term. Significant at P < 0.05. Values in bold indicate significant effects. Circles represent one individual zebrafish from batch 1 and squares represent zebrafish from batch 2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.