FIGURE

Fig. 5

ID

ZDB-FIG-230913-15

Publication

Simonian et al., 2023 - Methylenetetrahydrofolate reductase deficiency and high dose FA supplementation disrupt embryonic development of energy balance and metabolic homeostasis in zebrafish

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Fig. 5

mthfr deficiency in zebrafish reduces microglia and increases apoptosis in the optic tectum. (A–D) Representative bright-field images of NR+ staining at 4 dpf for microglia in (A) cas9, (B) 4gRNA, (C) WT and (D) mthfr −/− mutant zebrafish (mthfr −/−, HSC194). 80× magnification, scale bar = 100 μm. (E) Number of NR+ cells at 4 dpf. n = 8–14 larvae/group. Analyzed by one-way ANCOVA with ‘batch’ as a covariate. Significance at ^*^*P < 0.01 and ^*^*^*P < 0.001. (F, G) Representative fluorescent images of 3 dpf (F) WT and (G) mthfr −/− zebrafish stained with AO. (H) Number of AO+ cells at 3 dpf. White dotted line outlines area of quantification. 80× magnification, scale bar = 200 μm. Analyzed by Student’s t-test. Significant at ^*^*^*P < 0.001. All values are mean ± SEM. Circles represent one individual zebrafish from batch 1 and squares represent zebrafish from batch 2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.