High-throughput quantitative histological analysis. A Representative pictures of all cell-types analyzed for all the diets and timepoints with cells of interest in dashed black lines per each group. B Redundancy analysis to examine the effect of time on the histological parameters analyzed. The x axis separated the samples by timepoints and explained 5.76% of the variation observed. The link of time and variation of the histological parameters was not significant (p = 0.066) C Redundancy analysis to examine the effect of diet on the histological parameters analyzed. The x axis separated the samples of butyrate fed fish from saponin and control fed fish and explained 5.86% of the variation explained. The y axis separated saponin fed fish from control fed fish and explained 5.07% of the variation observed. The link of time and variation of the histological parameters was significant (p = 0.036). The top 10 most distinctive histological parameters are depicted in black arrows. The direction of the arrows correlate with the dietary intervention and the length of the arrows represents the strength of the correlation. The boxplots around the RDA depicted the absorptive capacity and the percentage area of cells of interest compared to the total gut area per diet and timepoint. *p ≤ 0.05, one-way ANOVA test or Kruskal–Wallis test after testing for normality on data distribution by Shapiro–Wilk test. No false discovery rate performed. Whiskers: min. to max. shall all points with median
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