Figure 9

SCD1 inhibition decreases hepatic lipid accumulation and membrane remodeling after exposure to DDE. Zebrafish transgenic larvae at 3 days post-fertilization were co-exposed to a specific SCD1 inhibitor (A939572 5 µM) and to DDE (10 µM) during 48 h. (a) For the steatosis score evaluation, larvae were euthanized following exposure and fixed, and steatosis scores (based on lipid fluorescence intensity, lipid droplet density, and area in the liver) were calculated based on Nile red staining and image-based automated analysis after confocal microscopy acquisition. Values are mean +/− SEM; with the number of batches and larvae indicated in the table underneath the graph *** p < 0.001, by comparison with control group using two-way ANOVA and Bonferroni tests. (b) Membrane order was assessed in liver cells of zebrafish larvae after staining with di-4-ANEPPDHQ—a membrane order-sensitive fluorescent probe—and analyzed by confocal fluorescence microscopy. Membrane order in membranes of zebrafish liver was measured by computing the generalized polarization (GP) factor. Changes in GP values (ΔGP) were expressed as the difference between individual larva GP value and the mean GP measured in control larvae (DMSO). Values are mean +/− SEM for n ≥ 3 batches per conditions. * p < 0.05, ** p < 0.01, by comparison with all groups using ANOVA and Newman–Keuls test.