Figure 8

DDE exposure-induced membrane remodeling in the liver of zebrafish larvae. Membrane order characteristic of membrane remodeling was assessed in liver of zebrafish larvae at 3 dpf after exposure for 48 h to DDE (0.1/1/10 µM) or to control vehicle. Zebrafish larvae were stained with di-4-ANEPPDHQ—a membrane order-sensitive fluorescent probe—and analyzed by confocal fluorescence microscopy. Membrane order in membranes of zebrafish liver was measured by computing the generalized polarization (GP) factor. (a) Representative liver images of control vehicle (DMSO) and DDE (10 µM) treatment (magnification ×400). The liver is delimited by the dotted lines. (b) Changes in GP values (ΔGP) were expressed as the difference between individual larva GP value and the mean GP calculated in control larvae (DMSO). Values are the mean ± standard error of the mean (SEM) of at least 20 larvae. * p < 0.05 by comparison with the control group using ANOVA and Dunnett’s test.