FIGURE

Figure 7

ID

ZDB-FIG-230228-180

Publication

Le Mentec et al., 2023 - A New In Vivo Zebrafish Bioassay Evaluating Liver Steatosis Identifies DDE as a Steatogenic Endocrine Disruptor, Partly through SCD1 Regulation

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Figure 7

Assessment of potential mitochondrial dysfunction induced by DDE. Zebrafish larvae at 3 dpf were exposed to DDE (from 1 nM to 10 µM) or to control vehicle during 48 h. (a) Impact of DDE on the mRNA expression of several genes involved in mitochondrial metabolism. mRNA expression was evaluated by RT-qPCR gene expression analysis by using the microfluidic Fluidigm-Biomark technology. Data are expressed relative to mRNA levels measured in DMSO control larvae, set at 0 (log 2 fold change) (n ≥ 8). (b) Evaluation of respiration after DDE exposure of zebrafish larvae. Measurements on zebrafish larvae were realized on a Seahorse XFe24 Analyzer of mitochondrial oxygen consumption. Values are the mean of oxygen consumption rate (OCR, in pmol of O₂/min) ± SEM measured from at least four larvae per condition. Values are the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, by comparison with control group using ANOVA and Dunnett’s test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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