FIGURE

Figure 3

ID

ZDB-FIG-221031-29

Publication

Basheer et al., 2022 - Zebrafish Model of Severe Combined Immunodeficiency (SCID) Due to JAK3 Mutation

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Figure 3

Effect of SCID-derived JAK3 mutations on zebrafish larval lymphopoiesis. Analysis of immune and blood cells in 28 dpf wild-type (wt/wt) and mutant (mdu9/mdu9) jak3 larvae using RT-PCR with primers specific for T cell receptor (TCR) β-chain (v(d)j-cβ vb1.5, vb12, vb14.5) and B cell Ig heavy chain (igvh1, igvh4) rearrangements along with actb as a control (n = 2) (A), noting that RT-negative controls yielded no products, or qRT²-PCR analysis with the indicated hematopoietic cell markers (B). Data is represented as relative fold-change compared to homozygous wild-type (wt/wt) larvae with mean and SEM shown in red and level of statistical significance of normalized Cq values (relative to control actb) indicated (** p < 0.01, * p < 0.05, ns not significant; n = 3).

Expression Data

Genes:	cd4-1 cd8a cd79a hbba1 ighd ighm ighv1-1 ighv4-6 lmo2 nccrp1 nkl.3 nkl.4 pax5
Fish:	WT jak3^mdu9/mdu9
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jak3^mdu9/mdu9
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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