FIGURE

Figure 1

ID

ZDB-FIG-221018-84

Publication

Park et al., 2022 - N-myc downstream regulated gene 1 (ndrg1) functions as a molecular switch for cellular adaptation to hypoxia

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Figure 1

Ndrg1a is not required for the differentiation of the pronephric duct and ionocytes.

(A, B) Lateral views of 24 hpf WT, standard MO-injected, ndrg1a MO-injected and ndrg1a^-/- mutant embryos labeled using (A) wholemount in situ hybridization to detect the mRNA distribution of slc20a1a, slc12a3, atp1a1a, and kcnj1a.1 (n=3–4 experiments with an average of 36 embryos per riboprobe) and (B) immunolabeling with anti-ATP1A1A or anti-GFP in Tg[enpep:GFP] transgenic line (n=3–4 experiments with an average of 40 embryos per antibody). Annotations: arrows point to signal in the pronephric duct; arrowheads: ionocytes. Scale bars 300 μm.

Expression Data

Genes:	atp1a1a.2 kcnj1a.1 slc12a3 slc20a1a
Antibody:	Ab1-atp1a
Fish:	ndrg1a^mbc1/mbc1 AB + MO2-ndrg1a
Knockdown Reagent:	MO2-ndrg1a
Anatomical Terms:	ionocyte pronephric duct pronephric duct anterior region pronephric duct medial region pronephric duct posterior region
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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