FIGURE

Fig. 4

ID

ZDB-FIG-220922-37

Publication

Denans et al., 2022 - An anti-inflammatory activation sequence governs macrophage transcriptional dynamics during tissue injury in zebrafish

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Fig. 4

a Representative confocal images (maximum projection of a 30 μm z-stack) of an HCR-FISH for dusp1 in a neuromast (10 larvae per condition, 30 neuromasts total and 3 biological replicates). b, c Representative confocal images (projection of a 30 μm z-stack) of HCR-FISH within the effector macrophages (arrows) for (b) il10ra and (c) fgl2a (12 larvae per conditions, 36 neuromasts total and 3 biological replicates) d, e Quantifications of the percentage of HCR + effector macrophages (MΦ) for (d) il10ra and (e) fgl2a (12 larvae per conditions, 36 neuromasts total and 3 biological replicates). For all graphs, data are represented as mean ± SD. f Integrated UMAP of the six datasets for the il10ra mutant. Cluster names are labeled on the UMAP. g Split UMAP per condition (il10ra ± and il10ra-/-). h Line plots representing the average expression for each time point between the il10ra mutant (cyan) and the sibling (red) from the scRNA-seq integrated dataset. i Model of independent activation of the three anti-inflammatory pathways in effector macrophages during the HC regeneration time course. Resting macrophages are represented in cyan. Purple arrows represent the independent induction of each macrophage activation state.

Expression Data

Genes:	dusp1 fgl2a il4r.1 il10ra manf odc1
Fish:	gl22Tg il10ra^psi71/+; gl22Tg il10ra^psi71/psi71; gl22Tg
Conditions:	chemical treatment by environment: neomycin chemical treatment by environment: neomycin
Anatomical Term:	neuromast macrophage
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gl22Tg il10ra^psi71/+; gl22Tg il10ra^psi71/psi71; gl22Tg
Conditions:	chemical treatment by environment: neomycin chemical treatment by environment: neomycin
Observed In:	macrophage interleukin-4-mediated signaling pathway macrophage polyamine biosynthetic process neuromast macrophage
Stage:	Day 5

Phenotype Detail

Acknowledgments

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