FIGURE

Fig. 1

ID

ZDB-FIG-220908-23

Publication

Ma et al., 2022 - Ercc2/Xpd deficiency results in failure of digestive organ growth in zebrafish with elevated nucleolar stress

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Fig. 1

Ercc2/Xpd is crucial for zebrafish larvae development

(A) Representative images of ercc2/xpd mutants and siblings at the indicated stages. Magnified images of digestive organs are shown on the right. Red arrows indicate microphthalmia in mutants. White arrowheads point to collapse the intestine in mutants. sb, swimming bladder; y, yolk. Scale bars, 200 μm.

(B) Survival curves of ercc2/xpd mutants and siblings. n = 100 larvae each.

(C) Fluorescence images of Tg(fabp10a:dsRed; ela3l:GFP) and ET33J1 fish showing endodermal organs of ercc2/xpd mutants and siblings at 5 and 7 dpf. Lateral view, anterior to the left. Scale bar, 100 μm.

(D–F) Quantification of liver, pancreas, intestine tube area in ercc2/xpd mutants and siblings at 5 and 7 dpf. n ≥ 12. Data are presented as mean ± SD, Student’s t test, ∗∗∗∗, p < 0.0001.

(G) Whole-mount in situ hybridization and fluorescence images showing mesoderm- and ectoderm-derived organs of ercc2/xpd mutants and siblings at 5 dpf. Scale bars, 100 μm. See also Figures S1 and S2.

Expression Data

Genes:	anxa5b cdh17 ddx4 DsRed EGFP gh1
Fish:	AB ercc2^{whu312/whu312} gz15Tg s843Tg ercc2^whu312/+; gz15Tg ercc2^{whu312/whu312}; s843Tg ercc2^{whu312/whu312}; sqet33J1Et
Anatomical Terms:	adenohypophysis blood vasculature intestine liver pancreas primordial germ cell pronephric duct swim bladder
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ercc2^{whu312/whu312} gz15Tg s843Tg ercc2^whu312/+; gz15Tg ercc2^{whu312/whu312}; gz15Tg ercc2^{whu312/whu312}; s843Tg ercc2^{whu312/whu312}; sqet33J1Et
Observed In:	blood vasculature exocrine pancreas eye gut intestine liver mandibular arch skeleton pancreas swim bladder whole organism yolk
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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