Fig. 4
- ID
- ZDB-FIG-220908-26
- Publication
- Ma et al., 2022 - Ercc2/Xpd deficiency results in failure of digestive organ growth in zebrafish with elevated nucleolar stress
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Ercc2/Xpd-deficient intestinal endothelial cells exhibit loss of cell polarity and impaired proliferation (A) Tg(Nkx2.2a:EGFP) marked intestinal endocrine cells in ercc2/xpd mutants and siblings at 5 dpf. Scale bars, 100 μm. (B) Alcian blue staining showed intestinal goblet cells (left) and quantification of their numbers (right) in ercc2/xpd mutants and siblings at 5 dpf. Scale bars, 100 μm. Data are presented as mean ± SD. Student’s t test, ∗∗∗∗, p < 0.0001. (C) EdU incorporation assay and immunostaining of the mitotic marker phospho-histone H3 revealed reduced the proliferation of intestinal endothelial cells (IECs) in ercc2/xpd mutants compared to siblings at 5 dpf. Scale bars, 100 μm. (D) Quantification of percentages of EdU-positive IECs or pH3-positive cells in different regions of the intestine. i.b., intestinal bulb; m.i., mid-intestine; p.i., posterior intestine. Data are presented as mean ± SD, Student’s t test, ∗∗∗∗, p < 0.0001, ∗∗∗, p < 0.001. See also Figure S4D. (E) The TUNEL assay showed no detectable apoptotic signals in IECs in ercc2/xpd mutants and siblings compared to the DNase-I-treated positive control at 7 dpf. Scale bars, 100 μm. See also Figure S5A. (F) Immunostaining of γ-H2A.X revealed no increased apoptosis or DNA instability in IECs in ercc2/xpd mutants compared to siblings at 7 dpf. Scale bars, 100 μm. |
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| Stage: | Day 5 |
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| Stage: | Day 5 |