Fig. 1
- ID
- ZDB-FIG-220607-37
- Publication
- Sánchez-Iranzo et al., 2022 - Strength of interactions in the Notch gene regulatory network determines patterning and fate in the notochord
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(A) Schematic representation of notochord development. At 18–19 hpf most of the notochord is composed of coin-shaped precursor cells. During the following 8 hours, progressively, in an antero-posterior order, coin-shaped precursor cells begin their differentiation into sheath cells and vacuolated cells. (B) Airyscan confocal section of a zebrafish notochord at 19 hpf using the rcn3:lyn-mNeonGreen transgenic line. (C) Transmission electron microscopy of a zebrafish notochord at 19 hpf. (D) Magnification of boxed area in (C). (E) (Top) Schematic representation of the model for a Lateral Induction Network shows a pair of cells where the ligand in one cell activates NICD release in the neighboring cell. NICD activates ligand expression in its own cell. (Bottom) Representative simulation of this network applied to an array of cells unidimensionally arranged. (F) (Top) Schematic representation of the model for a Lateral Inhibition Network shows a pair of cells where the ligand in one cell activates NICD release in the neighboring cell. NICD activates the expression of the repressor, which in turn inhibits ligand expression. (Bottom) Simulation of this network applied to an array of cells unidimensionally arranged. (G–L) Maximal intensity projection of Airyscan confocal sections of a zebrafish tail at 22 hpf. (J–L) Magnification of boxed area in (G). n = 10 fish. (L) Intensity profile across a horizontal line in panel (L). (M) jag1a:mScarlet and tp1:GFP expression levels across a 1 μm thick horizontal line on a single plane of the image shown in J. Scale bars, 1 μm (D) 10 μm (B, C, J), 100 μm (G). |