ZFIN ID: ZDB-FIG-210210-84
Zhou et al., 2020 - Identification of Potentially Relevant Genes for Excessive Exercise-Induced Pathological Cardiac Hypertrophy in Zebrafish. Frontiers in Physiology   11:565307 Full text @ Front. Physiol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 1 Figure 1. Excessive exercise leads to pathological cardiac hypertrophy. (A,B) Representative images from control (A) and excessively-exercised zebrafish hearts (B) after 4 weeks of static and excessive-exercise treatment. (C–F) Representative pictures of Masson’s trichrome-stained ventricular tissues of control (C,D) and excessively-exercised zebrafish hearts (E,F). Blue area indicates fibrosis. (D,F) Are enlargements of the rectangle area in (C,E), respectively. Scale bar = 100 μm. (G) Quantitative analysis of Masson staining positive myocardial fibrosis area using Image J. ∗p < 0.05 by unpaired Student’s t-test. n = 3. (H,I) TEM images of control (H) and excessively exercised heart tissue (I). Scale bar = 5 μm. (J) RT-qPCR analysis of the expression of mitochondrial functional markers. ∗p < 0.05, ∗∗p < 0.01 by unpaired Student’s t-test. (K) Echocardiographic analysis of zebrafish after excessive exercise and in the static control (LVPW, LV posterior wall thickness). Error bars indicate SEM. n = 4. (L) The maximal oxygen consumption (MO2) of zebrafish after excessive exercise and in the control group was analyzed. n = 8. (M) RT-qPCR analysis of the expression of pathologic and physiological hypotrophy-related marker genes. ∗p < 0.05, ∗∗p < 0.01 by unpaired Student’s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyc1 AB water flow Adult heart RTPCR
eif4ea AB water flow Adult heart RTPCR
map2k2a AB water flow Adult heart RTPCR
mapk3 AB water flow Adult heart RTPCR
mt-nd1 AB water flow Adult heart RTPCR
mt-nd2 AB water flow Adult heart RTPCR
mt-nd5 AB water flow Adult heart RTPCR
mt-nd6 AB water flow Adult heart RTPCR
myh7 AB water flow Adult heart RTPCR
nppa AB water flow Adult heart RTPCR
nppb AB water flow Adult heart RTPCR
pik3r1 AB water flow Adult heart RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB standard conditions Adult cardiac muscle cell cardiac myofibril disorganized, abnormal
Adult cardiac muscle cell mitochondrion-associated adherens complex disorganized, abnormal
Adult cardiac muscle decreased contractility, abnormal
Adult heart decreased functionality, abnormal
Adult heart increased size, abnormal
Adult myocardium fibrotic, abnormal
AB water flow Adult heart cyc1 expression decreased amount, abnormal
Adult heart eif4ea expression decreased amount, abnormal
Adult heart map2k2a expression decreased amount, abnormal
Adult heart mapk3 expression decreased amount, abnormal
Adult heart mt-nd1 expression decreased amount, abnormal
Adult heart mt-nd2 expression decreased amount, abnormal
Adult heart mt-nd5 expression decreased amount, abnormal
Adult heart mt-nd6 expression decreased amount, abnormal
Adult heart myh7 expression increased amount, abnormal
Adult heart nppa expression increased amount, abnormal
Adult heart nppb expression increased amount, abnormal
Adult heart pik3r1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Physiology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Physiol.