Gastrulation marker goosecoid (gsc) is not affected by VitE deficiency. Gsc expression at 6 hpf in the neuroectoderm of the dorsal embryonic shield, as indicated by red fluorescence in (A) E+ embryos (n = 9/9) and (B) E− embryos (n = 8/8). Arrow indicates dorsal region of the embryo. Scale bar represents 500 μm; representative embryos are shown. Figure panels were generated with the BZ- × 700 microscope, processed with BZ-X Analyzer Software with image adjustments equally applied across time points in Adobe Photoshop v21.2.1.