Figure 8

Histological analysis of 24 hpf zebrafish embryos with morphological defects associated with VitE status. Hematoxylin and eosin staining of E+ and E− embryos at 24 hpf was used to evaluate morphological defects, including fore- (F) and mid- (M), and hind- (H) brain ventricle inflation, somite formation and notochord vacuolation. Transverse section of E+ embryos had tear-drop shaped-F with eyes to each side (A), whereas E− embryos had a neural lumen, but improperly inflated-F (B). Serial transverse sections of E+ embryos showed normal M ventricle inflation with distinct hinge-points, indicated by *(C). E− embryos (D) had reduced M ventricle inflation with similar hinge points. E+ and E− embryos had similar H inflation leading to the neural tube in the trunk (E, F). Sagittal sections of the trunk indicate defined somite epithelial boundaries in E+ embryos (G) and indistinct epithelial boundaries in E− somitic cells (H). Notochord vacuolation appeared normal in both E+ (I) and E− (J) embryos. Scale bar represents 50 μm; representative embryos are shown. Figure panels were generated with the BZ- × 700 microscope, processed with BZ-X Analyzer Software with image adjustments equally applied across time points in Adobe Photoshop, v21.2.1.