ZFIN ID: ZDB-FIG-201003-140
Head et al., 2020 - Vitamin E is necessary for zebrafish nervous system development. Scientific Reports   10:15028 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 5-9 somites to Prim-5

Figure 4

Midbrain-hindbrain boundary formation shown by pax2a expression is dysregulated by VitE deficiency. Pax2a expression in early optic stalk (os), midbrain-hindbrain boundary (mhb) and otic placode (op) in 12 hpf embryos, lateral views (A, B); arrow indicates dorsal region. At 12 hpf, E+ embryos (C) had defined mhb and op borders (n = 8/8), while E− embryos (D) had diffuse mhb and op borders (n = 8/12 assessed). Shown are representative E+ embryo with mhb 25 μm wide and op 49 μm apart, while representative E− embryo measurements were mhb 42 μm wide and op 63 μm apart. At 24 hpf, in E+ (E) and E− embryos (F) pax2a was expressed in the os, mhb, otic vesicles (ov) and spinal cord neurons (sn). Distance between os and mhb, a measure of first brain ventricle inflation, were greater in a representative E+ (91 μm) relative to an E− (80 μm) embryo. E+ embryo (E) spinal cord neurons at the same fluorescence exposure had significantly increased pax2a signal (n = 9/9), as compared with E− embryos (n = 6/9). Scale bar represents 100 μm; representative embryos are shown. Figure panels were generated with the BZ- × 700 microscope, processed with BZ-X Analyzer Software with image adjustments equally applied across time points in Adobe Photoshop v21.2.1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a SPF 5-D standard conditions 5-9 somites anterior neural rod ISH
5-9 somites midbrain hindbrain boundary neural rod ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 optic stalk ISH
Prim-5 otic vesicle ISH
Prim-5 spinal cord neuron ISH
SPF 5-D nutrient decreased 5-9 somites anterior neural rod ISH
5-9 somites midbrain hindbrain boundary neural rod ISH
Prim-5 spinal cord neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
SPF 5-D nutrient decreased 5-9 somites anterior neural rod pax2a expression spatial pattern, abnormal
5-9 somites midbrain hindbrain boundary neural rod pax2a expression spatial pattern, abnormal
Prim-5 midbrain-hindbrain boundary development disrupted, abnormal
Prim-5 spinal cord neuron pax2a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.