ZFIN ID: ZDB-FIG-201003-144
Head et al., 2020 - Vitamin E is necessary for zebrafish nervous system development. Scientific Reports   10:15028 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 5-9 somites to Prim-5

Figure 6

Notochord collagen markers col2a1a and col9a2 affected by dietary treatment. At 12 hpf, col2a1a expression was localized in the developing notochord of E+ (A, n = 5/5) and E− (B, n = 6/6) embryos. Arrow indicates dorsal region of the embryo. At 24 hpf, the notochord sheath in E+ and E− embryos showed col2a1a (C, D) and col9a2 (G, H) expression with a wavy notochord phenotype observed in both groups. The wavy notochord was more apparent when viewing dorsally (E, F, I, J) relative to lateral view (C, D, G, H). At 24 hpf, ~ 25% of E+ embryos (E, n = 4/12) showed a wavy col2a1a expression, while ~ 66% of E− embryos (F, n = 6/9) were obviously wavy; similarly a wavy col9a2 expression was observed in 33% of E+ embryos (I, n = 3/10), while ~ 60% of E− embryos had a wavy expression (J, n = 5/8). Scale bar represents 500 μm (C–J) and 100 μm (A, B); representative embryos are shown. Figure panels were generated with the BZ- × 700 microscope, processed with BZ-X Analyzer Software with image adjustments equally applied across time points in Adobe Photoshop. This figure was created with Adobe Photoshop v21.2.1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a SPF 5-D standard conditions 5-9 somites notochord ISH
Prim-5 notochord ISH
SPF 5-D nutrient decreased 5-9 somites notochord ISH
Prim-5 notochord ISH
col9a2 SPF 5-D standard conditions 5-9 somites notochord ISH
Prim-5 notochord ISH
SPF 5-D nutrient decreased 5-9 somites notochord ISH
Prim-5 notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
SPF 5-D nutrient decreased 5-9 somites notochord col2a1a expression spatial pattern, abnormal
5-9 somites notochord col9a2 expression spatial pattern, abnormal
Prim-5 notochord col2a1a expression spatial pattern, abnormal
Prim-5 notochord col9a2 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.