ZFIN ID: ZDB-FIG-201003-138
Head et al., 2020 - Vitamin E is necessary for zebrafish nervous system development. Scientific Reports   10:15028 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: Shield to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Figure 2

Ttpa signal localized throughout early embryo and brain ventricle borders regardless of VitE status. Ttpa expression in E+ and E− embryos is indicated with red fluorescence; dorsal direction is indicated by arrow (AD). At 6 hpf (dorsal shield stage, A, B), Ttpa expression was present throughout the animal poles [E+ embryos, n = 5/5 (n = number of animals with the observed defect/total number of animals observed)], E− embryos, n = 6/6). At 12 hpf (90% epiboly, C, D), ttpa expression was present both in the embryo and in the yolk syncytial layer (E+ embryos, n = 6/6; E− embryos, n = 7/8 ), arrow indicates anterior region of the embryo. At 24 hpf (E, F), Ttpa expression was localized in the brain ventricle borders and within cells of the fore (f), mid- (m), and hindbrain (h). Arrows indicate the midbrain-hindbrain boundary where diencephalic ventricle expansion was altered; *Represent inflation in E+ embryos (E, n = 6/6) or lack thereof in E− embryos (F, n = 3/7). Scale bar represents 500 μm (A–D) and 50 μm (E, F); representative embryos are shown. Figure panels were generated with the BZ- × 700 microscope, processed with BZ-X Analyzer Software with image adjustments equally applied across time points in Adobe Photoshop v21.2.1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ttpa SPF 5-D standard conditions Shield whole organism ISH
5-9 somites whole organism ISH
5-9 somites yolk syncytial layer ISH
Prim-5 ventricular system anatomical margin ISH
SPF 5-D nutrient decreased Shield whole organism ISH
5-9 somites whole organism ISH
5-9 somites yolk syncytial layer ISH
Prim-5 ventricular system anatomical margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
SPF 5-D nutrient decreased Prim-5 choroid plexus third ventricle morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.