FIGURE

Fig. 2

ID

ZDB-FIG-200817-22

Publication

Bottiglione et al., 2020 - Zebrafish IL-4-like Cytokines and IL-10 Suppress Inflammation but Only IL-10 Is Essential for Gill Homeostasis

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Fig. 2

Increased levels of proinflammatory cytokines in il4/13a;b^−/− larvae. (A and B) qPCR analysis showing levels of il1β, il6, tnfα, ifng1–2, and mpx mRNA in wildtype, il4/13a^−/−, il4/13b^−/−, and il4/13a;b^−/− 5 dpf larvae. Dots indicate individual fish. Gene expression was normalized to the expression of β−actin. Error bars represent SEM; n > 3, *p < 0.05, **p < 0.01, ***p < 0.001. (C) Representative image of mpx in situ hybridization in wildtype and il4/13a^−/− 3 dpf larvae (n = 9–10). Purple dots indicate mpx⁺ cells. Scale bar, 100 μm. (D) Quantification of mpx⁺ cells in wildtype and il4/13a^−/− larvae head region. Dots indicate individual fish.

Expression Data

Genes:	ifng1 il1b il6 mpx tnfa
Fish:	WT il4^umc18/umc18 il13^umc17/umc17 il4^umc18/umc18; il13^umc17/umc17
Anatomical Terms:	gill neutrophil
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	il4^umc18/umc18 il13^umc17/umc17 il4^umc18/umc18; il13^umc17/umc17
Observed In:	gill
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Immunol.