FIGURE

Fig. 6

ID

ZDB-FIG-200817-26

Publication

Bottiglione et al., 2020 - Zebrafish IL-4-like Cytokines and IL-10 Suppress Inflammation but Only IL-10 Is Essential for Gill Homeostasis

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Fig. 6

Prolonged inflammatory response in il10^e46/e46 gills following R848 stimulation. (A) qPCR analysis showing transcript levels of il1β, il6, tnfα, and ifng1–2 in gills from wildtype and il10^e46/e46 animals following R848 challenge for 1 and 8 h. Gene expression was normalized to the expression of β−actin. Fold change is shown. Error bars represent SEM; n > 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Representative images of sections (n = 3) of gills from wildtype and il10^e46/e46 animals (n = 3) stained with H&E following 8-h R848 challenge. Epithelial cell alteration in the lamellae (black arrows) and gill filament (stars) are indicated in gills from il10^e46/e46 animals. Scale bar, 50 μm. (C) Quantification of gill tissue damage in wildtype and il10^e46/e46 animals following R848 challenge.

Expression Data

Genes:	ifng1 il1b il6 tnfa
Fish:	WT il10^e46/e46
Condition:	chemical treatment: chemical substance
Anatomical Term:	gill
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT il10^e46/e46
Condition:	chemical treatment: chemical substance
Observed In:	gill gill filament gill lamella epithelial cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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