FIGURE

Fig. 1

ID

ZDB-FIG-200327-4

Publication

Ye et al., 2019 - High fat diet induces microbiota-dependent silencing of enteroendocrine cells

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Fig. 1

Identification of neurod1+ enteroendocrine cells (EECs) in zebrafish.

( A) Confocal projection of zebrafish EECs marked by the TgBAC(neurod1:EGFP) transgenic line. ( B) Confocal projection of zebrafish EECs marked by Tg(neurod1:RFP). TgBAC(cldn15la:GFP) marks intestinal epithelial cells. ( C) Confocal image of zebrafish EECs marked by TgBAC(neurod1:EGFP) transgenic line. ( C?) Subpanel image of zebrafish enterocyte marked by Tg(fabp2:DsRed). Note that neurod1+ EECs do not express the enterocyte marker fabp2. ( D) Schematic diagram of 6 dpf larval zebrafish intestine. The anterior region of the intestine that is largely responsible for nutrient absorption is highlighted in yellow. ( E?F) Confocal image of neurod1+ EECs stained for PYY (E,) and CCK ( F). ( E??F?) Zoom view of PYY and CCK positive EECs. ( G?H) Confocal image of neurod1+ EECs expressing somatostatin [marked by Tg(sst2:DsRed) in G] and proglucagon hormones [marked by Tg(gcga:EGFP) in H]. ( G??H?) Zoom view of sst2 and gcga positive EECs. ( I?J) Quantification of PYY+ (n = 7) and CCK+ (n = 4) EECs in 6 dpf zebrafish intestines.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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