FIGURE

Fig. 8

ID

ZDB-FIG-200327-35

Publication

Ye et al., 2019 - High fat diet induces microbiota-dependent silencing of enteroendocrine cells

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Fig. 8

High fat feeding induced EEC silencing is microbiota dependent.

( A?B) Confocal images of 6 dpf zebrafish intestines from conventionalized (CV) and germ free (GF) larvae following 10 hr of high fat (HF) feeding. EECs are marked with Tg(neurod1:RFP) and the apical lumen of intestine is marked with Tg(gata5:lifeAct-EGFP). ( A??B?) Zoom view of EECs from CV and GF zebrafish following HF feeding. Yellow arrows in B? indicate EEC apical extensions in HF fed GF zebrafish. ( C?F) Representative images of the EEC calcium response toward palmitate stimulation in CV and GF Tg(neurod1:Gcamp6f)larvae with or without 6 hr of HF feeding. ( G?H) Quantification of the EEC calcium response to palmitate stimulation represented in C-F. ( I) Quantification of the EEC morphology score in CV and GF zebrafish larvae with or without 10 hr of HF feeding represented in A and B. ( J) Quantification of xpb1+ EECs (%) in CV and GF Tg(neurod1:RFP); Tg(ef1?:xbp1?-GFP)zebrafish larvae with or without 6 hr HF feeding. ( K) Quantification of NF-?B+ EECs (%) in CV and GF Tg(neurod1:RFP); Tg(NFkB:EGFP) zebrafish larvae with or without 10 hr HF feeding. Student t-test was used in G,H and one-way ANOVA with post-hoc Tukey test was used in I-K for statistical analysis. *p<0.05, **p<0.01, ****p<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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