Fig. 5

( A) Representative image of zebrafish after 6 hr of high-fat (HF) feeding, or HF feeding followed by 1, 3.5, and 20 hr of recovery in fresh egg water. ( B) EEC palmitate-induced calcium response using Tg(neurod1:Gcamp6f) transgenic zebrafish after 6 hr of HF feeding, or HF feeding followed by 1, 3.5, and 20 hr of recovery. ( C–E) Confocal projection of representative EECs (magenta) in Tg(neurod1:RFP) zebrafish under control conditions or 8 hr of HF feeding and HF fed zebrafish following 20 hr of recovery. ( F) Confocal projection of representative EECs of Tg(neurod1:RFP); Tg(gata5:lifeAct-EGFP) in HF fed zebrafish following 20 hr of recovery. Yellow arrows indicate EECs’ apical extensions. ( G) Change of Gcamp6f relative fluoresence intensity in response to palmitate stimulation in HF fed, and HF fed zebrafish following 1, 3.5, and 20 hr of recovery. ( H) Quantification of EEC palmitate response in control and HF fed zebrafish following 20 hr of recovery. ( I) Quantification of EEC morphology in control, HF fed and HF fed zebrafish following 20 hr of recovery. ( J) Representative image of HF fed Tg(neurod1:Kaede) zebrafish following 20 hr recovery. Kaede+ EECs are photoconverted at 6 hr post HF feeding before and after recovery. ( K) Quantification of the percentage of newly generated EECs (green Kaede only) in 3d post UV photoconversion, 20 hr post UV photoconversion and in HF fed zebrafish photoconverted before 20 hr recovery. Student t-test was used in H and one-way ANOVA with post-hoc Tukey test was used in I for statisitical analysis. ***p<0.001, ns p>0.05, not signficantly different.