FIGURE

Fig. 1-S2

ID

ZDB-FIG-200327-6

Publication

Ye et al., 2019 - High fat diet induces microbiota-dependent silencing of enteroendocrine cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1-S2

Analysis of EEC lifespan in zebrafish larvae using single dose EdU labeling.

EdU was injected into the pericardiac sac region of 5 dpf TgBAC(neurod1:EGFP) zebrafish using previously described methods ( Ye et al., 2015). Zebrafish were fixed at 1 hr, 4 hr, 20 hr, 30 hr, 45 hr, 54 hr, 7 days (168 hr) and 15 days post EdU injection. ( A–D) Confocal images of EdU fluorescence staining in the TgBAC(neurod1:EGFP) zebrafish intestine. ( E) Quantification of the percentage of EdU+ EECs in zebrafish intestine following EdU tracing. t = 0 (n = 6), t = 1 hr (n = 8), t = 4 hr (n = 5), t = 20 hr (n = 6), t = 30 hr (n = 11), t = 45 hr (n = 9), t = 54 hr (n = 6), t = 168 hr (n=5). No EdU+ EECs could be detected until 30 hr post EdU injection and some EdU+ EECs remained 15 days post EdU injection. ( F) Schematic of our working model of EEC lifespan.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife