SGDR is preserved in MTZ-treated Tg(dat:NTR-CFP) larvae. Ai, DAPI (white)-labeled horizontal section of Tg(dat:NTR-CFP) zebrafish brain with high-magnification images of the caudal hypothalamus taken from a (Aii) DMSO-treated and (Aiii) MTZ-treated fish. OB = olfactory bulbs, Te = telencephalon, Pr = pretectum, DC = diencephalon, Hc = caudal hypothalamus, LC = locus coeruleus, TeO = optic tectum, Ce = cerebellum, Re = retina. Nomenclature as per Godoy et al. (2015). CFP expression is depicted in magenta. Scale bars = 100 μm (i) and 10 μm (ii). B, Average swimming episode duration (in seconds) calculated for 50 episodes (10 per fish for five fish) at 3 and 5 dpf for DMSO-treated Tg(dat:NTR-CFP) fish and at 5 dpf only for MTZ-treated Tg(dat:NTR-CFP) fish. One-way ANOVA (F(2,147) = 26.8, p = 1 × 10−10) followed by two-tailed unpaired Student’s t test (3- to 5-dpf DMSO, p = 3 × 10−9; 3- to 5-dpf MTZ, p = 0.0031; and 5- to 5-dpf MTZ, p = 2 × 10−10); *p < 0.0166, indicating significance with Bonferroni’s multiple-comparisons correction. C, Results from Peak20–40 detection algorithm for autocorrelation functions of each extracellular recording at 3 and 5 dpf for DMSO-treated Tg(dat:NTR-CFP) fish and at 5 dpf for MTZ-treated Tg(dat:NTR-CFP) fish under control (blue) and strychnine (red) conditions (Ci). Attenuation of Peak20–40 detection computed as 1 – (Peak20–40Strychnine/Peak20–40Ctrl) for each fish. N = 60 episodes (10 per fish for six fish) for 5-dpf Tg(dat:NTR-CFP) fish (Cii). For Ci, two-tailed paired Student’s t test (3-dpf DMSO Ctrl-Str, p = 0.019; 5-dpf DMSO Ctrl-Str, p = 0.0091; 5-dpf MTZ Ctrl-Str, p = 0.0036). For Cii, one-way ANOVA followed (F(2,26) = 3.98, p = 0.0409) followed by two-tailed unpaired Student’s t test (3- to 5-dpf DMSO, p = 0.0102; 3-dpf DMSO to 5-dpf NTR, p = 0.0398; and 5-dpf WT to 5-dpf NTR, p = 0.8155). Top horizontal bars display result of tests between the two groups at each ends of the bars. Error bars represent S.E.M.
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