ZFIN ID: ZDB-FIG-191122-4
Park et al., 2019 - 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish. Scientific Reports   9:1138 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 2 Lipid metabolism- and mitochondrial homeostasis-associated gene expression, and oxygen consumption analysis in larvae. Relative mRNA expression of genes associated with lipid metabolism include sterol regulatory element-binding protein 1 (srebp1), fatty acid synthase (fasn), srebp2lipoprotein lipase (lpl), and carnitine-palmitoyltransferase I (cpt1) ( A). Oil red O staining in control, kdsrI105R mutant, and kdsrI105R mutant siblings after fasnall treatment at 7 dpf; 1 µM fasnall treatment was performed from 4 dpf to 7 dpf ( B). Images shown are representative of at least 10 in total. Scale bar in B = 100um. Average oxygen consumption rate was measured from 7 groups (3 larvae per group) of control and mutant siblings for 30 minutes ( C). Relative mRNA expression of genes associated with mitochondrial homeostasis include dynamin related protein 1 (drp1), mitofusin 1 (mfn1), optic atrophy type 1 (opa1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (pgc1a), and NADH-ubiquinone oxidoreductase chain1 (nd1) ( D). Error bars indicate standard deviation of the mean. *P ≤ 0.05, **P ≤ 0.005.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cpt1aa AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
dnm1l AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
fasn AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
lpl AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
mfn1b AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
mt-nd1 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
opa1 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
ppargc1a AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
srebf1 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
srebf2 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU chemical treatment by environment: N-(1-benzylpyrrolidin-3-yl)-5,6-dimethylthieno[2,3-d]pyrimidin-4-amine Days 7-13 liver lipid increased amount, exacerbated
Days 7-13 liver lipid increased amount, exacerbated
kdsrmuz106/muz106 (AB/TU) standard conditions Days 7-13 liver lipid increased amount, abnormal
Days 7-13 respiratory gaseous exchange by respiratory system increased rate, abnormal
Days 7-13 whole organism cpt1aa expression increased amount, abnormal
Days 7-13 whole organism fasn expression increased amount, abnormal
Days 7-13 whole organism lpl expression increased amount, abnormal
Days 7-13 whole organism mfn1b expression increased amount, abnormal
Days 7-13 whole organism mt-nd1 expression increased amount, abnormal
Days 7-13 whole organism opa1 expression increased amount, abnormal
Days 7-13 whole organism ppargc1a expression increased amount, abnormal
Days 7-13 whole organism srebf1 expression increased amount, abnormal
Days 7-13 whole organism srebf2 expression increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.