ZFIN ID: ZDB-FIG-191122-4
Park et al., 2019 - 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish. Scientific Reports   9:1138 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 2 Lipid metabolism- and mitochondrial homeostasis-associated gene expression, and oxygen consumption analysis in larvae. Relative mRNA expression of genes associated with lipid metabolism include sterol regulatory element-binding protein 1 (srebp1), fatty acid synthase (fasn), srebp2lipoprotein lipase (lpl), and carnitine-palmitoyltransferase I (cpt1) ( A). Oil red O staining in control, kdsrI105R mutant, and kdsrI105R mutant siblings after fasnall treatment at 7 dpf; 1 µM fasnall treatment was performed from 4 dpf to 7 dpf ( B). Images shown are representative of at least 10 in total. Scale bar in B = 100um. Average oxygen consumption rate was measured from 7 groups (3 larvae per group) of control and mutant siblings for 30 minutes ( C). Relative mRNA expression of genes associated with mitochondrial homeostasis include dynamin related protein 1 (drp1), mitofusin 1 (mfn1), optic atrophy type 1 (opa1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (pgc1a), and NADH-ubiquinone oxidoreductase chain1 (nd1) ( D). Error bars indicate standard deviation of the mean. *P ≤ 0.05, **P ≤ 0.005.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cpt1aa AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
dnm1l AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
fasn AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
lpl AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
mfn1b AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
mt-nd1 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
opa1 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
ppargc1a AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
srebf1 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
srebf2 AB/TU standard conditions Days 7-13 whole organism RTPCR
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU chemical treatment by environment: N-(1-benzylpyrrolidin-3-yl)-5,6-dimethylthieno[2,3-d]pyrimidin-4-amine Days 7-13 liver lipid increased amount, exacerbated
Days 7-13 liver lipid increased amount, exacerbated
kdsrmuz106/muz106(AB/TU) standard conditions Days 7-13 liver lipid increased amount, abnormal
Days 7-13 respiratory gaseous exchange by respiratory system increased rate, abnormal
Days 7-13 whole organism cpt1aa expression increased amount, abnormal
Days 7-13 whole organism fasn expression increased amount, abnormal
Days 7-13 whole organism lpl expression increased amount, abnormal
Days 7-13 whole organism mfn1b expression increased amount, abnormal
Days 7-13 whole organism mt-nd1 expression increased amount, abnormal
Days 7-13 whole organism opa1 expression increased amount, abnormal
Days 7-13 whole organism ppargc1a expression increased amount, abnormal
Days 7-13 whole organism srebf1 expression increased amount, abnormal
Days 7-13 whole organism srebf2 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.