FIGURE

Fig. S3

ID
ZDB-FIG-190618-29
Publication
Park et al., 2019 - 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. S3

Supporting Fig. 3. The kdsrcri mutant generated by CRISPR gene targeting and sphingolipids profiling. Wild type and the kdsrcri mutant images at 7 dpf (A), Scale bar=0.25 mm. H & E staining and ORO staining in control (B, left), mutant at 7 dpf (B, right). Scale bar=50µm. Relative amount of sphingolipids from 30 control siblings and 30 kdsrI105R mutant larvae siblings at 7 dpf (C) and 30 control siblings and 30 kdsrcri mutant larvae siblings at 7 dpf (D). Relative mRNA expression in sphingolipid salvage pathway components in control siblings and kdsrcri mutants at 7 dpf (E). CRISPR/Cas9 RNA injection generated premature stop codon in exon3 of kdsr in the kdsrcri mutant by 31 bps deletion and 16 bps insertion (F). * P≤0.05, ** P≤0.005.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.