FIGURE

Fig. 3

ID

ZDB-FIG-190723-84

Publication

Yang et al., 2019 - Cul4a promotes zebrafish primitive erythropoiesis via upregulating scl and gata1 expression

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Downregulation of zebrafish <italic>cul4a</italic> has no effect on primitive myelopoiesis or definitive hematopoiesis.

aPu.1 was analyzed by WISH at 24 hpf in embryos injected with CoMO or cul4a-MO. b Relative expression of pu.1 mRNA was analyzed by qRT-PCR in embryos injected with CoMO or cul4a-MO. c, d Expression of pu.1 in control and cul4a^−/−, cul4b^−/−, and double knockout mutants was assayed by WISH (c) and by qRT-PCR (d). e, fMpo was analyzed by WISH (e) and qRT-PCR (f) at 24 hpf in embryos injected with CoMO or cul4a-MO. g–jrunx1 and c-myb expression were analyzed by WISH (g, i) or qRT-PCR (h, j) in embryos injected with CoMO or cul4a-MO. k, l Expression of runx1 in control and cul4a^−/−, cul4b^−/−, and double knockout embryos. The number in the top right-hand corner indicates the phenotypic embryos/total embryos. qRT-PCR experiments were performed in triplicate. All scale bars represent 250 μm

Expression Data

Genes:	mpx myb runx1 spi1b
Fish:	WT cul4a^{zf3203/zf3203} cul4b^{zf3204/zf3204} WT + MO2-cul4a cul4a^{zf3203/zf3203}; cul4b^{zf3204/zf3204}
Knockdown Reagent:	MO2-cul4a
Anatomical Terms:	granulocyte hematopoietic cell myeloid cell
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.