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ZDB-IMAGE-190723-125

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Yang et al., 2019 - Cul4a promotes zebrafish primitive erythropoiesis via upregulating scl and gata1 expression

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Fig. 3 Downregulation of zebrafish <italic>cul4a</italic> has no effect on primitive myelopoiesis or definitive hematopoiesis.

aPu.1 was analyzed by WISH at 24 hpf in embryos injected with CoMO or cul4a-MO. b Relative expression of pu.1 mRNA was analyzed by qRT-PCR in embryos injected with CoMO or cul4a-MO. c, d Expression of pu.1 in control and cul4a^−/−, cul4b^−/−, and double knockout mutants was assayed by WISH (c) and by qRT-PCR (d). e, fMpo was analyzed by WISH (e) and qRT-PCR (f) at 24 hpf in embryos injected with CoMO or cul4a-MO. g–jrunx1 and c-myb expression were analyzed by WISH (g, i) or qRT-PCR (h, j) in embryos injected with CoMO or cul4a-MO. k, l Expression of runx1 in control and cul4a^−/−, cul4b^−/−, and double knockout embryos. The number in the top right-hand corner indicates the phenotypic embryos/total embryos. qRT-PCR experiments were performed in triplicate. All scale bars represent 250 μm

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