FIGURE

Fig. 4.

ID

ZDB-FIG-190723-1982

Publication

Reuter et al., 2019 - Fgf3 is crucial for the generation of monoaminergic cerebrospinal fluid contacting cells in zebrafish

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Fig. 4.

Quantification of the number of oxt-,avp- and cort-expressing cells in the hypothalamus of fgf3^t24152 mutants and fgf3 morphants at 72 hpf. (A–L) Light microscopic pictures of wild type (+/+) and homozygous fgf3^t24152 mutant (−/−) siblings as well as fgf3 morphants (MO) and uninjected control siblings (UC) processed for RNA in situ hybridisation. The posterior cells expressing avp are more affected by impaired fgf3 than the anterior ones. Ventral views, anterior to the left. Scale bar: 30 µm. (M–R) Quantifications of oxt-, avp- and cort-positive cells in fgf3^t24152 mutants, fgf3 morphants and control siblings. Cell clusters used for the analyses are indicated by the lines in A, E and I. Tukey boxplots show median, 25–75% percentile, IQR whiskers and outliers. n=number of analysed individuals, +/−, heterozygous fgf3^t24152 mutants. *P>0.05, **P>0.01, ***P>0.001.

Expression Data

Genes:	avp oxt sst7
Fish:	AB TU fgf3^{t24152/t24152} (TU) AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Anatomical Terms:	hypothalamus hypothalamus neuron
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf3^{t24152/t24152} (TU) AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Observed In:	hypothalamus
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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