Fig. 8.

Hierarchical clustering of hypothalamic Fgf-signalling pathway genes and downstream targets analysed in fgf3^t24152 mutants (−/−) and wildtypes (+/+) at 3 and 7 dpf. (A) Scheme illustrating sample preparation and groups used for RNA sequencing. Micrographs show the dissected hypothalamic area (ventral view, anterior to the left) collected from wild types (blue tubes) and fgf3^t24152 mutants (green tubes) at 3 (light tubes) and 7 dpf (dark tubes). For each group, three independent tissue collections were performed resulting in a total of 12 samples processed for RNA sequencing. Scale bar: 500 µm. (B,D) List of fgfs, fgfrs, Fgf downstream target genes and Fgf-signalling components expressed (base mean ≥10) in the hypothalamus of −/− and +/+ embryos. All genes except shc2 pass the base mean threshold in all four groups. shc2 does not pass the base mean threshold in the 3 dpf −/− group. (C,E) Heat maps of differentially regulated (base mean ≥10, fold change ≥1.5) fgfs, fgfrs and Fgf downstream target genes (C) as well as Fgf-signalling components (E) in the hypothalamus of −/− and +/+ embryos. Columns represent z-score of mean values of replicates for each analysed group and rows depict individual genes. Colour key displays z-score ranging from ≥−1.5 to ≤1.5. Colour intensity represents expression levels of a gene for each group with blue or yellow indicating low or high expression.