Fig. S5

Quantification of the number of serotonergic cells in the intermediate (i.)/posterior (p.) clusters and of dopaminergic cells in the DC 4/5/6 and DC 7 clusters in the hypothalamus of fgf3  morphants at 4 dpf. (AH) Confocal maximum intensity projections from uninjected control (UC) and morpholino injected (MO) siblings immuno stained for 5-HT (green) and TH1 (magenta) shown as single channels and merged.  C and  G show boxed areas in  B and  F, respectively, with adjusted brightness and contrast to reveal the faint TH1 immunoreactive cells of the DC 7 cluster.  (L, M) Light microscopic pictures of fgf3 morphants and uninjected control siblings processed for RNA in situ hybridisation for th2 expressed by dopaminergic cells intermingled with TH1 positive cells in the DC7 cluster. Insets show high magnifications of boxed areas. Ventral views, anterior to the left. Scale bars in  A,E,C,G, 10 μm; in  L, 30 μm.  (I-K, N) Quantifications of 5-HT, TH1 and th2 positive cells in control and morphant siblings. The number of serotonergic cells was counted in the i./p. clusters as indicated by the line in  A. The number of dopaminergic (TH1 and th2) cells was counted in the DC 4/5/6 and DC 7 clusters as indicated by the lines in  B and  C, respectively. Tukey boxplots showing median, 25-75% percentile, IQR whiskers and outliers. n = number of analysed individuals.