FIGURE

Fig. 5.

ID

ZDB-FIG-190723-1983

Publication

Reuter et al., 2019 - Fgf3 is crucial for the generation of monoaminergic cerebrospinal fluid contacting cells in zebrafish

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Fig. 5.

The size of the hypothalamic nkx2.4b domain is significantly reduced in fgf3^t24152 mutants and fgf3 morphants. (A–L) Light microscopic pictures showing expression of nkx2.4b in wild type (+/+) and homozygous fgf3^t24152 mutant (−/−) siblings as well as fgf3 morphants (MO) and uninjected control siblings (UC) at 36, 48 and 72 hpf visualised by RNA in situ hybridisation. Outlines of semi-automated measurements of hypothalamic area are highlighted in blue. Ventral views, anterior to the left. Scale bars: 30 µm. (M–R) Area measurements (pixels) in fgf3^t24152 mutants, fgf3 morphants and control siblings. Tukey boxplots show median, 25–75% percentile, IQR whiskers and outliers. n=number of analysed individuals +/−, heterozygous fgf3^t24152 mutants. *P>0.05, ***P>0.001.

Expression Data

Gene:	nkx2.4b
Fish:	AB TU fgf3^{t24152/t24152} (TU) AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Anatomical Term:	hypothalamus
Stage Range:	Prim-25 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf3^{t24152/t24152} (TU) AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Observed In:	hypothalamus
Stage Range:	Prim-25 to Protruding-mouth

Phenotype Detail

Acknowledgments

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