FIGURE

Fig. 1

ID

ZDB-FIG-190722-14

Publication

Zhao et al., 2019 - Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10-Imp3-Imp4 complex to nucleolus

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Fig. 1

Zebrafish Sas10 and Mpp10 are nucleolar proteins and display dynamic expression patterns during early embryogenesis. (A and B) Diagram showing the genomic structures of the zebrafish sas10 gene on chromosome 11 (A) and mpp10 gene on chromosome 7 (B). Red bar, exons; blue line, introns. Numbers above each exon and under each intron indicate their length (bp). (C) Western blot of Sas10, Mpp10, Def, Fib, Bhmt and α-Tub in total protein (Total), cytoplasmic fraction (CP), nucleoplasmic fraction (NP) and nucleolar fraction (NO) from adult livers. (D–F) Co-immunostaining of Sas10 and Fib (D), Mpp10 and Fib (E), or Mpp10 and Sas10 (F) in the adult liver. Big box shows the high magnification view of the nucleus in the small box. Nuclei were stained with DAPI; bar, 50 μm. (G and H) WISH using the fabp10a, fabp2, trypsin; insulin probes on 4dpf-old WT, sas10^Δ2 (s^Δ2) (G) and mpp10^Δ10 (m^Δ10) (H) embryos. The number of total embryos genotyped (as denominator) and the number of embryos exhibiting the displayed phenotype (as numerator) are shown at the bottom.

Expression Data

Genes:	fabp2 fabp10a ins mphosph10 prss1 utp3
Antibody:	Ab1-fbl
Fish:	AB WT mphosph10^zju10/zju10 utp3^zju8/zju8
Anatomical Terms:	endocrine pancreas exocrine pancreas intestine liver liver nucleolus
Stage Range:	Day 4 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mphosph10^zju10/zju10 utp3^zju8/zju8
Observed In:	exocrine pancreas intestine liver
Stage:	Day 4

Phenotype Detail

Acknowledgments

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