ZFIN Image: Zhao et al., 2019, Fig. 1

Image

Figure Caption

Fig. 1

Zebrafish Sas10 and Mpp10 are nucleolar proteins and display dynamic expression patterns during early embryogenesis. (A and B) Diagram showing the genomic structures of the zebrafish sas10 gene on chromosome 11 (A) and mpp10 gene on chromosome 7 (B). Red bar, exons; blue line, introns. Numbers above each exon and under each intron indicate their length (bp). (C) Western blot of Sas10, Mpp10, Def, Fib, Bhmt and α-Tub in total protein (Total), cytoplasmic fraction (CP), nucleoplasmic fraction (NP) and nucleolar fraction (NO) from adult livers. (D–F) Co-immunostaining of Sas10 and Fib (D), Mpp10 and Fib (E), or Mpp10 and Sas10 (F) in the adult liver. Big box shows the high magnification view of the nucleus in the small box. Nuclei were stained with DAPI; bar, 50 μm. (G and H) WISH using the fabp10a, fabp2, trypsin; insulin probes on 4dpf-old WT, sas10^Δ2 (s^Δ2) (G) and mpp10^Δ10 (m^Δ10) (H) embryos. The number of total embryos genotyped (as denominator) and the number of embryos exhibiting the displayed phenotype (as numerator) are shown at the bottom.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.