FIGURE

Fig. 4

ID

ZDB-FIG-180912-17

Publication

Kinoshita et al., 2018 - Functional roles of the Ripply-mediated suppression of segmentation gene expression at the anterior presomitic mesoderm in zebrafish

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Fig. 4

Expression of segmentation genes in the PSM is not fully suppressed in tbx6^+/−; ripply1^−/− mutants. Expression patterns of mesp-ba (A–C), mesp-bb (D–F), her1 (G–I), deltaC (J–L), tbx6 (M–O), pcdh8/papc (P–R), fgf8a (S–U), tbx18 (V–X), myod (Y–A′), and uncx4.1 (B′–D′) in sibling, tbx6^+/+; ripply1^−/−, and tbx6^+/−; ripply1^−/− embryos were examined by whole-mount in situ hybridization at the 18–22-somite stage. A line shows the ectopic expression of segmentation genes in the paraxial mesoderm. The arrowhead shows the polarized expression of somite polarity markers at the anterior PSM. Scale bar in D′ is 100 μm. All of the photos were taken at the same magnification. Higher magnification of the photos (S–D′) is shown in the Supplementary Fig. 7.

Expression Data

Genes:	dlc fgf8a her1 mespba mespbb myod1 pcdh8 tbx6 tbx18 uncx4.1
Fish:	WT ripply1^{sud101/sud101} ripply1^{sud101/sud101}; tbx6^ti1/+
Anatomical Terms:	segmental plate somite
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ripply1^{sud101/sud101} ripply1^{sud101/sud101}; tbx6^ti1/+
Observed In:	segmental plate somite
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 152, Kinoshita, H., Ohgane, N., Fujino, Y., Yabe, T., Ovara, H., Yokota, D., Izuka, A., Kage, D., Yamasu, K., Takada, S., Kawamura, A., Functional roles of the Ripply-mediated suppression of segmentation gene expression at the anterior presomitic mesoderm in zebrafish, 21-31, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.