header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180824-19
Collins et al., 2018 - Pitx2c orchestrates embryonic axis extension via mesendodermal cell migration. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to 1-4 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to 1-4 somites

Fig. 2

MZpitx2c mutants are correctly patterned but exhibit defects in spatial organization.

(a–g) Analysis of goosecoid (gsc) (a), noto/flh (b, c), eve1 (d), wnt8a (e), tbx16 (f), and sox17 (g) expression by in situ hybridization. Expression of gsc (dorsal organizer) at 80% epiboly is indistinguishable between MZpitx2c mutants and wild types (a). At 60% epiboly, MZpitx2c mutants display an expanded domain of noto expression (axial mesoderm) (b) and subsequently a shorter and wider notochord at the one somite stage (ss) (c). A higher magnification of the boxed regions in panel c is shown in the lower panels. The eve1 expression domain (ventral mesoderm) is expanded dorsally in MZpitx2c mutants (d) (outlined by dashed lines and indicated by arrows in the vegetal pole views), and no obvious changes were observed in wnt8a expression (embryonic margin) (e). Expression of tbx16 (paraxial mesoderm) is disrupted in the dorsal region of MZpitx2c mutants (f). Expression of sox17 (endoderm) reveals defects in dorsal forerunner cell migration (arrowheads) in 80% epiboly MZpitx2c mutants (g). a-c and e-g: dorsal views, animal pole to the top; a-b: animal pole views, dorsal to the right of the image; d: lateral views, dorsal to the right of the image; d-e: vegetal pole views, dorsal to the right of the image. ‘n’ refers to the number of embryos with the expression pattern shown over the total number of embryos analyzed. AP, animal pole; D, dorsal; V, ventral. Scale bars, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
eve1 WT standard conditions Shield presumptive ventral mesoderm ISH
pitx2ups6/ups6 standard conditions Shield presumptive ventral mesoderm ISH
gsc WT standard conditions 75%-epiboly prechordal plate ISH
pitx2ups6/ups6 standard conditions 75%-epiboly prechordal plate ISH
noto WT standard conditions Shield shield ISH
1-4 somites notochord ISH
pitx2ups6/ups6 standard conditions Shield shield ISH
1-4 somites notochord ISH
sox17 WT standard conditions 75%-epiboly endoderm ISH
pitx2ups6/ups6 standard conditions 75%-epiboly endoderm ISH
tbx16 WT standard conditions Shield presumptive paraxial mesoderm ISH
pitx2ups6/ups6 standard conditions Shield presumptive paraxial mesoderm ISH
wnt8a WT standard conditions Shield margin ISH
pitx2ups6/ups6 standard conditions Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pitx2ups6/ups6 standard conditions Shield presumptive paraxial mesoderm tbx16 expression spatial pattern, abnormal
Shield presumptive ventral mesoderm eve1 expression increased distribution, abnormal
Shield shield noto expression increased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly forerunner cell group mislocalised, abnormal
75%-epiboly forerunner cell group cell migration process quality, abnormal
1-4 somites notochord noto expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife