FIGURE
Fig. 5
- ID
- ZDB-FIG-180706-56
- Publication
- Cao et al., 2018 - Metalloproteinase Adamts16 Is Required for Proper Closure of the Optic Fissure
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Fig. 5
Gene expression changes in adamts16-morphant eyes. (A–L) Expression of various genes essential for key cellular events of OF closure examined by in situ hybridization. tbx5: dorsal retina marker; vax2: ventral retina marker; aldh1a2: RA-synthesizing enzyme and dorsal retina marker; aldh1a3: RA-synthesizing enzyme and ventral retina marker; foxC1: POM marker and regulator; pax2: OF marker and regulator. (M) Heatmap of differentially expressed genes in 36-hpf control, MO1-, and MO2-morphant eyes analyzed by high-throughput sequencing. Left: Downregulated genes. Right: Upregulated genes. Green circles highlight cell cycle regulators and purple circles highlight cell death regulators. (N–O) In situ hybridization of fgf8 in control and MO1-morphant heads. Red arrowheads point to the midbrain-hindbrain boundary. Red arrows point to the OS. (P) Quantification of coloboma prevalence shows that coloboma phenotype of MO1- or MO2-morphants can be rescued by fgf8-MO coinjection. Scale bar: 100 μm.
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Expression Data
| Genes: | |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage: | Prim-25 |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage Range: | Prim-25 to Protruding-mouth |
Phenotype Detail
Acknowledgments
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Full text @ Invest. Ophthalmol. Vis. Sci.